Identification and Genotyping of the Etiological Agent of Tuberculous Lymphadenitis in Ethiopia

Background: In Ethiopia; little has been done to assess how Mycobacterium bovis has contributed to human tuberculosis; though the population routinely consumes unpasteurized milk and raw meat. The aim of this study was to determine the proportion of M. tuberculosis and M. bovis as etiological agents of tuberculous lymphadenitis (TBLN). Methods: Patients with lymphadenopathy (n = 171) were included in a cross-sectional study at Butajira Hospital; Southern Ethiopia. Lymph node biopsies were cultured. Patients' HIV status was identified. DNA from positive cultures was tested by PCR to identify M. bovis and M. tuberculosis. Isolates were genotyped by multiplex ligation-dependent probe amplification (MLPA) assay. Results: Among 171 patients; 156 had culture results. Of these; 107 (69) were positive for M. tuberculosis complex (MTC). Six of the 10 HIV-positive patients were culture positive. M. tuberculosis specific sequences were identified in the DNA of each of 100 samples as assessed by RD10 targeted PCR; and each of the 95 isolates exhibited the M. tuberculosis specific TbD1 deletion by MLPA analysis. No M. bovis was identified. These results indicate that all the isolates were modern M. tuberculosis strains. Furthermore; MLPA studies confirmed that 42of the isolates showed the Haarlem genotype and 12displayed sequences compatible with INH resistance. No mutations conferring resistance to ethambutol or rifampicin were detected. Conclusions: Our data showed that M. tuberculosis strains had common characteristics with strains causing pulmonary TB; which appears to be the main etiological agent of TBLN

Voies de drainage du groupe des lymphonoeuds intertracheobronchiques. Revision anatomique a partir de l'injection de la plevre diaphragmatique de foetus et de sujets adultes

Le but de cette etude anatomique etait de preciser les differentes voies de drainage du volumineux groupe des lymphonoeuds intertracheobronchiques. Les vaisseaux lymphatiques de la plevre diaphragmatique de 23 foetus et de 57 sujets adultes ont ete injectes avec une masse de Gerota modifiee de facon a opacifier a partir d'afferences diaphragmatiques; le volumineux groupe des lymphonoeuds intertracheobonchiques et ses differentes voies de drainage. Chacun des temps de la dissection a ete decrit et photographie. Le drainage efferent apparait essentiellement paratracheal ascendant et plus souvent droit que gauche. Une voie moins connue des anatomistes classiques; la chaine tracheo-oesophagienne thoracique droite; a ete individualisee. Le role et la topographie du groupe des lymphonoeuds sus-bronchiques gauches appendu au groupe des lymphonoeuds intertracheobronchiques; diversement apprecies par les anatomistes classiques; ont ete precises. Des liaisons directes ou indirectes avec le conduit thoracique et l'abdomen; moins connues; controversees; d'importance considerable en pathologie ont ete mises en evidence. Le volumineux groupe des lymphonoeuds intertracheobronchi-ques est un veritable carrefour de la lymphe. Ses voies de drainage sont essentiellement paratracheales ascendantes plus souvent droites que gauches

Difficultes et interets d'une exploitation optimale du role et de la signification des ganglions regionaux dans les cancers au Cameroun. [Mise au point]

Une etude de fiabilite de 209 dossiers medicaux adresses aux consultations de cancerologie radiotherapie a ete menee. Une enumeration de toutes les informations d'origine ganglionnaire regionale qui contribuent a la connaissance du statut tumoral; des facteurs de pronostic du bilan d'extension et de la classification de la maladie maligne avait pour but final d'operer le meilleur choix de la strategie therapeutique. Un rappel didactique de la signification des principaux parametres a ete realise. Un accent particulier a ete mis sur la necessite d'une bonne documentation des dossiers apres que les raisons de leur mauvaise tenue aient ete relevees

Primary peripheral lymphadenopathy in Jos; Nigeria

Surgical biopsy specimens of 129 enlarged perpheral nodes seen at Jos University Teaching Hospital over a 3-year period were analysed. Enlarged peripheral nodes due to primary sarcomas or carcinomas were excluded from the series. Tuberculosis and malignant lymphomas were mainly diagnosed

Review of lymph node biopsies: medical ward; Kamuzu Central Hospital; 1985-1988

A descriptive study of lymph node biopsies done on the medical ward of Kamuzu Central Hospital [KCH] in Lilongwe between 1985-1988 was undertaken to describe the histological diagnosis; the site of the lymph node biopsy; the sex; and age of the individual. There were 53 biopsies; 27 (51 percent) were tuberculosis [TB] adenitis; 10 (19 percent) were reactive nodes (? viral infection); 8 (15 percent) were Kaposi's Sarcoma; 7 (13 percent) were lymphoma or leukemia and one was normal. Kaposi's Sarcoma occured more significantly in males than females. TB adenitis was most frequently in the age group 21-40 years and it mostly occured in the cervical area. Further study may reveal a clinically useful relationship between lymph adenopathy; the various diagnosis and HIV status for the purposes of diagnosis and management